Figure 1.
Non-muscle Cells Are Involved in Nuclear Movement to the Periphery of Myofibers
(A) Representative transmitted image highlighting the interactions (yellow arrowheads) between non-muscle cells and myofibers at day 2 (no interaction, left) and day 5 (interactions, right). Bottom panels depict non-muscle cell (blue) and myofiber (dashed red) interaction as outlines from upper panels. Scale bars, 10 μm.
(B) Quantification of number of interactions between myofibers and non-muscle cell during muscle development. Data from three independent experiments with n = 19 myofibers quantified. Error bars correspond to SEM.
(C) Representative immunofluorescence image of in vitro culture containing myofibers and non-muscle cells stained either for α-smooth muscle actin (α-sm-actin, magenta), desmin (green), and DAPI (nucleus, blue). Scale bar, 10 μm.
(D) Quantification of number of myofibroblasts and other non-muscle cells per mm2, treated with vehicle or mitomycin, during muscle development. Data collected from three independent experiments with at least 12 areas of 322 mm2 for each condition. Error bars correspond to SEM.
(E) Representative immunofluorescence image of 10-day myofibers treated with vehicle, mitomycin, or mitomycin supplemented with untreated non-muscle cells and stained for F-actin (myofibrils, green) and DAPI (nucleus, blue). Scale bar, 10 μm.
(F) Quantification of peripheral nuclei in 10-day myofibers treated with vehicle, mitomycin, or mitomycin supplemented with untreated non-muscle cells. Data from three independent experiments were combined and error bars represent SEM from indicated number of nuclei (n) for each cohort. Unpaired t test was used to determine statistical significance, where ∗p < 0.05, ∗∗∗p < 0.001.