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. 2018 Jul 2;46(1):23–39.e5. doi: 10.1016/j.devcel.2018.06.006
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

Goat polyclonal anti-GFP Abcam Cat# ab6662; RRID: AB_305635
Rabbit anti-Bazooka Andreas Wordaz laboratory RRID: AB_2570125
Mouse anti-Armadillo DSHB Cat# 25E9.D7; RRID: AB_528104
Mouse anti-MMP1 DSHB Cat# 3A6B4; RRID: AB_579780
Mouse anti-MMP1 DSHB Cat# 3B8D12; RRID: AB_579781
Mouse anti-MMP1 DSHB Cat# 5H7B11; RRID: AB_579779
Mouse anti-Ubx DSHB Cat# FP3.38; RRID: AB_10805300
Goat anti-mouse Alexa fluor 546 Invitrogen Cat# A11030; RRID: AB_144695
Goat anti-rabbit Alexa fluor 546 Invitrogen Cat# A11035; RRID: AB_143051

Chemicals, Peptides, and Recombinant Proteins

Shield and Sang M3 insect medium Sigma S365
2% Fetal bovine serum Sigma F3018
Streptomycin/ampicillin antibiotics mix Invitrogen 15140-122
Ecdysone Sigma E9004
Insulin Sigma 5500
Methyl-cellulose Sigma M0387
Rock inhibitor Sigma Y-27632
Trypsin-EDTA Gibco 15400-054
DAPI Sigma D9542
Phalloidin-Atto 647N Sigma 65906

Experimental Models: Organisms/Strains

D. melanogaster: E-cadherin-GFP.
w; shg-GFP
Huang et al., 2009 N/A
D. melanogaster: Dumpy-YFP.
w[1118]; PBac{681.P.FSVS-1}dp[CPTI001769]
DGGR (Kyoto) Cat# 115238; RRID: DGGR 115238
D. melanogaster: Vkg-GFP.
w[∗]; P{PTT-un1}vkgG205
FlyTrap G205
D. melanogaster: Lanβ1-GFP.
w[∗];; LanB1-GFP/TM2
Sarov et al., 2016 N/A
D. melanogaster: Perlecan-GFP.
w[∗]P{w+mC=PTT-un1}ZCL1700
DGGR (Kyoto) Cat# 110807; RRID: DGGR_110807
D. melanogaster: Myosin-II-GFP.
sqhAX3; sqh-Sqh-GFP
Royou et al., 2002 N/A
D. melanogaster: MMP2 overexpression.
w[∗]; P{w[+mC]=UAS-Mmp2.P}2
Bloomington Cat# 58705; RRID: BDSC_58705
D. melanogaster: Timp overexpression.
w[∗]; P{w[+mC]=UAS-Timp.P}3
Bloomington Cat# 58708; RRID: BDSC_58708
D. melanogaster: UbxIa allele overexpression.
w[∗]; P{w[+mC]=UAS-UbxIa.C}36.2/TM3, Ser[1]
Bloomington Cat# 911; RRID: BDSC_911
D. melanogaster: RNAi of Bazooka.
w[∗];; bazNIG.5055R/TM3
NIG-Fly 5055R-1
D. melanogaster: RNAi of Ultrabithorax
w[1118]; P{GD5049}v37823
VDRC Cat# 37823; RRID: Flybase_FBst0462184
D. melanogaster: RokK116A allele overexpression
w[∗]; UAS.venus-RokK116A/TM6B
Simoes Sde et al., 2010 N/A
D. melanogaster: Dll-Gal4
w[∗]; Dll-Gal4/CyO
Calleja et al., 1996 N/A
D. melanogaster: nubbin-Gal4
w[∗]; nubbin-GAL4
Calleja et al., 1996 N/A
D. melanogaster: Ubx-Gal4
w[∗];; Ubx-Gal4/TM6B
GregoryGibson N/A
D. melanogaster: Fat8 mutant allele
w[∗]; FRT40A ftGrV/CyOGFP; AFZ/TM2
Mao et al., 2006 N/A
D. melanogaster: FatGrV mutant allele
w[∗]; FRT40A ft-GrV/CyOGFP; AFZ/TM2
Matakatsu and Blair, 2006 N/A

Software and Algorithms

Fiji Schindelin et al., 2012 RRID: SCR 002285