REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Antibodies | ||
Goat polyclonal anti-GFP | Abcam | Cat# ab6662; RRID: AB_305635 |
Rabbit anti-Bazooka | Andreas Wordaz laboratory | RRID: AB_2570125 |
Mouse anti-Armadillo | DSHB | Cat# 25E9.D7; RRID: AB_528104 |
Mouse anti-MMP1 | DSHB | Cat# 3A6B4; RRID: AB_579780 |
Mouse anti-MMP1 | DSHB | Cat# 3B8D12; RRID: AB_579781 |
Mouse anti-MMP1 | DSHB | Cat# 5H7B11; RRID: AB_579779 |
Mouse anti-Ubx | DSHB | Cat# FP3.38; RRID: AB_10805300 |
Goat anti-mouse Alexa fluor 546 | Invitrogen | Cat# A11030; RRID: AB_144695 |
Goat anti-rabbit Alexa fluor 546 | Invitrogen | Cat# A11035; RRID: AB_143051 |
Chemicals, Peptides, and Recombinant Proteins | ||
Shield and Sang M3 insect medium | Sigma | S365 |
2% Fetal bovine serum | Sigma | F3018 |
Streptomycin/ampicillin antibiotics mix | Invitrogen | 15140-122 |
Ecdysone | Sigma | E9004 |
Insulin | Sigma | 5500 |
Methyl-cellulose | Sigma | M0387 |
Rock inhibitor | Sigma | Y-27632 |
Trypsin-EDTA | Gibco | 15400-054 |
DAPI | Sigma | D9542 |
Phalloidin-Atto 647N | Sigma | 65906 |
Experimental Models: Organisms/Strains | ||
D. melanogaster: E-cadherin-GFP. w; shg-GFP |
Huang et al., 2009 | N/A |
D. melanogaster: Dumpy-YFP. w[1118]; PBac{681.P.FSVS-1}dp[CPTI001769] |
DGGR (Kyoto) | Cat# 115238; RRID: DGGR 115238 |
D. melanogaster: Vkg-GFP. w[∗]; P{PTT-un1}vkgG205 |
FlyTrap | G205 |
D. melanogaster: Lanβ1-GFP. w[∗];; LanB1-GFP/TM2 |
Sarov et al., 2016 | N/A |
D. melanogaster: Perlecan-GFP. w[∗]P{w+mC=PTT-un1}ZCL1700 |
DGGR (Kyoto) | Cat# 110807; RRID: DGGR_110807 |
D. melanogaster: Myosin-II-GFP. sqhAX3; sqh-Sqh-GFP |
Royou et al., 2002 | N/A |
D. melanogaster: MMP2 overexpression. w[∗]; P{w[+mC]=UAS-Mmp2.P}2 |
Bloomington | Cat# 58705; RRID: BDSC_58705 |
D. melanogaster: Timp overexpression. w[∗]; P{w[+mC]=UAS-Timp.P}3 |
Bloomington | Cat# 58708; RRID: BDSC_58708 |
D. melanogaster: UbxIa allele overexpression. w[∗]; P{w[+mC]=UAS-UbxIa.C}36.2/TM3, Ser[1] |
Bloomington | Cat# 911; RRID: BDSC_911 |
D. melanogaster: RNAi of Bazooka. w[∗];; bazNIG.5055R/TM3 |
NIG-Fly | 5055R-1 |
D. melanogaster: RNAi of Ultrabithorax w[1118]; P{GD5049}v37823 |
VDRC | Cat# 37823; RRID: Flybase_FBst0462184 |
D. melanogaster: RokK116A allele overexpression w[∗]; UAS.venus-RokK116A/TM6B |
Simoes Sde et al., 2010 | N/A |
D. melanogaster: Dll-Gal4 w[∗]; Dll-Gal4/CyO |
Calleja et al., 1996 | N/A |
D. melanogaster: nubbin-Gal4 w[∗]; nubbin-GAL4 |
Calleja et al., 1996 | N/A |
D. melanogaster: Ubx-Gal4 w[∗];; Ubx-Gal4/TM6B |
GregoryGibson | N/A |
D. melanogaster: Fat8 mutant allele w[∗]; FRT40A ftGrV/CyOGFP; AFZ/TM2 |
Mao et al., 2006 | N/A |
D. melanogaster: FatGrV mutant allele w[∗]; FRT40A ft-GrV/CyOGFP; AFZ/TM2 |
Matakatsu and Blair, 2006 | N/A |
Software and Algorithms | ||
Fiji | Schindelin et al., 2012 | RRID: SCR 002285 |