REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and Virus Strains
E.coli BL21 (DE3)	Agilent	200131
Chemicals, Peptides, and Recombinant Proteins
StrepTactin Sepharose High Performance	GE Healthcare	Cat# 28-9355-99
Polyethyleneimine	Polysciences	Cat# 24765-2
cOmplete™, EDTA-free Protease Inhibitor Cocktail	Roche	Cat# 4693116001
Tubulin Porcine	Cytoskeleton	Cat# T240-C
Tubulin Porcine TRITC	Cytoskeleton	Cat# TL590M
Tubulin Porcine HiLyte 488™	Cytoskeleton	Cat# TL488M
Tubulin Porcine HiLyte 647™	Cytoskeleton	Cat# TL670M
Tubulin Porcine	Cytoskeleton	Cat# T333P
GMPCPP	Jena Biosciences	Cat# NU-405L
GTP	Sigma	Cat# G8877
Glucose oxidase	Sigma	Cat# G7141
Catalase	Sigma	Cat# C9322
DTT	Sigma	Cat# R0861
k-casein	Sigma	Cat# C0406
Neutravidin	Invitrogen	Cat# A-2666
Taxol	Sigma	Cat# T7402
Colchicine	Sigma	Cat# C9754
Vinblastine sulfate salt	Sigma	Cat# V1377
Dolastatin	Sigma	Cat# D5566
Strep-GFP-CLASP2α full length	This study	N/A
Strep-GFP-CLASP1α full length	This study	N/A
Strep-GFP-CLASP2α IPNN	This study	N/A
Strep-GFP-CLASP2α 1-1176 (ΔCLIP-ID)	This study	N/A
Strep-GFP-CLASP2α 1-813 (TOG12-S)	This study	N/A
Strep-GFP-CLASP2α 734-1527 (S-TOG3-CLIP-ID)	This study	N/A
Strep-GFP-CLASP2α 734-1209 (S-TOG3)	This study	N/A
Strep-GFP-CLASP2α 734-813Λ1175-1527 (S-CLIP-ID)	This study	N/A
Strep-GFP-CLASP2α 1-261Λ524-1527 (ΔTOG2)	This study	N/A
Strep-GFP-CLASP2α 1-260Λ585-813 (TOG1-S)	This study	N/A
Strep-GFP-CLASP2α 261-813 (L-TOG2-S)	This study	N/A
Strep-GFP-CLASP2α 261-813 W339E (L-TOG2-S W339E)	This study	N/A
Strep-GFP-CLASP2α 261-813 R462E (L-TOG2-S R462E)	This study	N/A
Strep-GFP-CLASP2α 261-813 R504E (L-TOG2-S R504E)	This study	N/A
Strep-GFP-CLASP2α 295-813 (TOG2-S)	This study	N/A
Strep-GFP-CLASP2α 295-673 ΛEB3 1-200 (TOG2-EB3CH)	This study	N/A
Strep-GFP-chTOG 1-233ΛCLASP2α 601-813 (chTOG-TOG1-S)	This study	N/A
Strep-GFP-chTOG 262-495 ΛCLASP2α 601-813 (chTOG-TOG2-S)	This study	N/A
Strep-GFP-CLASP2α 734-813 (S)	This study	N/A
Strep-GFP-CLASP2α 281-1527 (ΔTOG1)	This study	N/A
Strep-GFP-CLASP2α 295-1149 (TOG2-S-TOG3)	This study	N/A
Strep-GFP-CLASP2α 295-813Λ1119-1527 (TOG2-S-CLIP-ID)	This study	N/A
Strep-GFP-CLASP2α 1-813Λ1119-1527(TOG1TOG2-S-CLIP-ID)	This study	N/A
Strep-GFP-MACF2 5455-5497ΛCLIP170 334-1232 (SxIPMACF-CCCLIP170)	This study	N/A
Strep-GFP-MCAK full length	This study	N/A
TOG1	This study	N/A
TOG2	This study	N/A
TOG3	This study	N/A
CLIP-ID	This study	N/A
mCherry-EB3 full length	Montenegro Gouveia et al., 2010	N/A
mCherry-EB3 ΔTail	Montenegro Gouveia et al., 2010	N/A
GFP-EB3 full length	Montenegro Gouveia et al., 2010	N/A
Deposited Data
Atomic coordinates and structure factors	PDBe	5NR4
Experimental Models: Cell Lines
Human: HEK293T	ATCC	CRL-11268
Monkey: COS7	ATCC	CRL-1651
MDA-MB231 EB3GFP	Bouchet et al., 2016	N/A
Oligonucleotides
siRNA targeting sequence: CLASP1# A: GCCATTATGCCAACTATCT	Mimori-Kiyosue et al., 2005	N/A
siRNA targeting sequence: CLASP2# A: GTTCAGAAAGCCCTTGATG	Mimori-Kiyosue et al., 2005	N/A
Recombinant DNA
Strep-GFP-CLASP2α 734-813 (S)	This study	N/A
Strep-GFP-CLASP2α 1-813 (TOG12-S)	This study	N/A
Strep-GFP-CLASP2α 1-260Λ585-813 (TOG1-S)	This study	N/A
Strep-GFP-CLASP2α 261-813 (L-TOG2-S)	This study	N/A
Strep-GFP-CLASP2α 261-813 R462E (L-TOG2-S R462E)	This study	N/A
Software and Algorithms
ImageJ	NIH	https://imagej.nih.gov/ij/
Metamorph	Molecular Devices	https://www.moleculardevices.com/products/cellular-imaging-systems/acquisition-and-analysis-software/metamorph-microscopy
Matlab	Mathworks	https://www.mathworks.com/
GraphPad Prism	GraphPad	https://www.graphpad.com/scientific-software/prism/
KymoResliceWide plugin	Eugene Katrukha	https://github.com/ekatrukha/KymoResliceWide
Multiscale Trend Analysis Matlab code	Eugene Katrukha	https://github.com/ekatrukha/MTA
Matlab code for Figures 6, 7, and S5	This study	https://doi.org/10.6084/m9.figshare.6260732