Table 1.
Rf, Linear regression data for the calibration curve of β-amyrin, β-sitosterol, lupeol and ursolic acid (n = 6).
| Parameters | β-Amyrin | β-Sitosterol | Lupeol | Ursolic Acid |
|---|---|---|---|---|
| Linearity range (ng/spot) | 100–1000 | 100–1000 | 100–1200 | 100–1200 |
| Regression equation | Y = 8.727x + 306.63 | Y = 8.843x + 1249.27 | Y = 3.892x + 324.92 | Y = 9.952x + 146.74 |
| Correlation (r2) coefficient | 0.9975 ± 0.0002 | 0.9983 ± 0.0005 | 0.9971 ± 0.0005 | 0.9987 ± 0.0004 |
| Slope ± SD | 8.727 ± 0.042 | 8.843 ± 0.049 | 3.892 ± 0.061 | 9.952 ± 0.089 |
| Intercept ± SD | 306.63 ± 11.53 | 1249.27 ± 20.955 | 324.92 ± 13.259 | 146.74 ± 11.432 |
| Standard error of slope | 0.017 | 0.020 | 0.025 | 0.036 |
| Standard error of intercept | 4.709 | 8.553 | 5.412 | 4.666 |
| Rf | 0.39 ± 0.004 | 0.25 ± 0.005 | 0.47 ± 0.004 | 0.23 ± 0.005 |
| LOD (ng) | 16.24 | 18.56 | 52.24 | 29.57 |
| LOQ (ng) | 49.21 | 56.26 | 158.32 | 89.61 |