Table 2.

Recovery as accuracy studies of the proposed method of β-amyrin, β-sitosterol, lupeol and ursolic acid (n = 6).

Stand. added to analyte (%)	Theor. conc. of stand. (ng/μL)	Method I						Method II
		β-Amyrin			β-Sitosterol			Lupeol			Ursolic acid
		Conc. found (ng/μL) ± SD	% RSD	% Recovery	Conc. found (ng/μL) ± SD	% RSD	% Recovery	Conc. found (ng/μL) ± SD	% RSD	% Recovery	Conc. found (ng/μL) ± SD	% RSD	% Recovery
0	200	199.91 ± 0.69	0.347	99.95	199.94 ± 1.21	0.608	99.97	199.83 ± 0.65	0.326	99.91	201.57 ± 0.41	0.204	100.78
50	300	298.94 ± 1.01	0.338	99.65	300.55 ± 1.96	0.652	100.18	300.69 ± 0.81	0.270	100.23	297.09 ± 0.52	0.176	99.03
100	400	400.56 ± 1.42	0.356	100.14	398.34 ± 2.71	0.682	99.58	398.31 ± 1.16	0.290	99.58	401.25 ± 0.71	0.178	100.31
150	500	496.13 ± 1.96	0.396	99.23	496.32 ± 3.49	0.703	99.26	499.04 ± 1.65	0.331	99.80	500.56 ± 0.92	0.184	100.11

Stand.: Standards; Theor.: Theoretical; Conc.: Concentration.