Table 3.
Precision of the proposed HPTLC Method-I & II (n = 6).
| Concentration of standard added (ng/spot) | β-Amyrin (Method I) |
β-Sitosterol (Method I) |
||||||
|---|---|---|---|---|---|---|---|---|
| Intra-day precision |
Inter-day precision |
Intra-day precision |
Inter-day precision |
|||||
| Average Conc. found ± SD | %RSD | Average Conc. found ± SD | %RSD | Average Conc. found ± SD | %RSD | Average Conc. found ± SD | %RSD | |
| 400 | 399.87 ± 0.81 | 0.205 | 398.72 ± 0.76 | 0.190 | 400.55 ± 1.42 | 0.355 | 399.42 ± 1.39 | 0.348 |
| 600 | 598.71 ± 1.33 | 0.222 | 597.56 ± 1.29 | 0.216 | 597.02 ± 2.16 | 0.362 | 595.89 ± 2.01 | 0.337 |
| 800 | 797.29 ± 1.87 | 0.235 | 793.86 ± 1.84 | 0.232 | 796.75 ± 2.99 | 0.375 | 793.36 ± 2.82 | 0.355 |
| Concentration of standard added (ng/spot) | Lupeol (Method II) | Ursolic acid (Method II) | ||||||
| Intra-day precision | Inter-day precision | Intra-day precision | Inter-day precision | |||||
| Average Conc. found ± SD | %RSD | Average Conc. found ± SD | %RSD | Average Conc. found ± SD | %RSD | Average Conc. found ± SD | %RSD | |
| 400 | 399.51 ± 0.95 | 0.238 | 396.94 ± 0.91 | 0.229 | 399.71 ± 0.52 | 0.131 | 397.19 ± 0.50 | 0.127 |
| 600 | 600.69 ± 1.51 | 0.251 | 598.12 ± 1.40 | 0.234 | 598.79 ± 0.81 | 0.136 | 595.77 ± 0.78 | 0.131 |
| 800 | 798.12 ± 2.09 | 0.262 | 795.55 ± 1.98 | 0.249 | 794.83 ± 1.17 | 0.147 | 791.81 ± 1.11 | 0.140 |