Table 5.

Robustness of the proposed HPTLC Method II at 300 ng/band (n = 6).

Optimization condition	Lupeol		Ursolic acid
	SD	%RSD	SD	%RSD
Mobile phase composition; (chloroform: methanol)
(97:3)	0.824	0.276	0.612	0.207
(95:5)	0.832	0.278	0.724	0.246
(98:2)	0.845	0.282	0.916	0.309
Mobile phase volume (for saturation)
(18 mL)	0.829	0.280	0.912	0.309
(20 mL)	0.851	0.287	0.924	0.314
(22 mL)	0.812	0.273	0.856	0.290
Duration of saturation
(10 min)	0.831	0.279	0.812	0.277
(20 min)	0.862	0.289	0.844	0.289
(30 min)	0.875	0.294	0.866	0.294