Figure 4.

PET, qPCR, and Histological Detection of hESC-ECP within Ischemic Hindlimbs at Early and Late Time Points

(A) Representative average time frames of nude (Crl:CD1-Foxn1^nu) mice with hindlimb ischemia up to 4 hr post-injection. *Injection site; ‡urinary bladder. (B) Estimation of cell retention within the injection site is shown; n = 5; **p ≤ 0.01, ***p ≤ 0.001, and ****p ≤ 0.0001 versus baseline by one-way ANOVA paired with post hoc Dunnett’s test. (C) qPCR quantification of human DNA in ischemic hindlimbs harvested at time points corresponding to imaging studies is shown. n = 6; *p < 0.05, **p ≤ 0.01, and ***p ≤ 0.001 versus baseline by one-way ANOVA with post hoc Dunnett’s test. (D) Immunofluorescence for human mitochondria (green) within (i) ischemic muscle 21 days post-injection (PI), (ii) ischemic muscle 4 hr post-injection, and (iii) human positive control (liver) is shown. Nuclei counterstained with DAPI (blue) are shown; images representative of n = 3. All data represent mean ± SEM.