Table 1.
Electrophysiological Characterization of HVPs during Ventriculogenesis In Vitro and In Vivo by Optical Mapping
| Parameter | NKX2.5- GFP Day 15+ In Vitro | NKX2.5-GFP Day 30+ In Vitro | NKX2.5-GFP 1+ Month Ex Vivo Kidney Graft | ES03 Day 15+ In Vitro | ES03 1+ Month Ex Vivo Kidney Graft |
|---|---|---|---|---|---|
| AP upstroke time (ms) | 128 ± 19 (n = 3) | 128 ± 24 (n = 3) | 129.7 ± 11.5 (n = 3) | 143 ± 9 (n = 3) | 105.0 ± 42.4 (n = 2) |
| AP 50% decay time (ms) | 73.5 ± 4.1 (n = 3) | 103.5 ± 5.5 (n = 3) | 93.9 ± 18.5 (n = 3) | 252 ± 41 (n = 3) | 151.6 ± 2.8 (n = 2) |
| APD50 (ms) | 202 ± 24 (n = 3) | 232 ± 30 (n = 3) | 223.6 ± 25.5 (n = 3) | 395 ± 48 (n = 3) | 256 ± 45 (n = 2) |
| APD90 (ms) | 374 ± 66 (n = 3) | 454 ± 10 (n = 3) | 378 ± 32 (n = 3) | 491 ± 91 (n = 3) | 304 ± 1 (n = 2) |
| Conduction velocity (cm/s) | 2.08 ± 0.91 (n = 3) | 2.41 ± 0.17 (n = 3) | 2.19 ± 0.14 (n = 3) | 3.35 ± 0.75 (n = 3) | 1.89 ± 0.58 (n = 2) |
Comparison of action potential parameters by optical mapping. The NKX2.5-GFP-labeled ESC line and ES03 cell line were employed for in vitro and ex vivo (i.e., kidney patch) analyses. Measurements were made during 1-Hz pacing.