Table 2.
Comparison of Calcium Transient Parameters between HVPs during Ventriculogenesis In Vitro and In Vivo by Optical Mapping
| Parameter | NKX2.5-GFP Day 15+ In Vitro | NKX2.5-GFP Day 30+ In Vitro |
|---|---|---|
| CaT upstroke time (ms) | 266 ± 5 (n = 3) | 234 ± 2 (n = 3) |
| CaT 50% decay time (ms) | 186 ± 23 (n = 3) | 255 ± 4 (n = 3) |
| CaT 50 (ms) | 452 ± 12 (n = 3) | 490 ± 2 (n = 3) |
The NKX2.5-GFP-labeled ESC line was employed for in vitro and ex vivo (i.e., kidney patch) analyses. All measurements made by optical mapping during 1-Hz pacing.