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. 2018 Feb 17;26(7):1644–1659. doi: 10.1016/j.ymthe.2018.02.012

Table 2.

Comparison of Calcium Transient Parameters between HVPs during Ventriculogenesis In Vitro and In Vivo by Optical Mapping

Parameter NKX2.5-GFP Day 15+ In Vitro NKX2.5-GFP Day 30+ In Vitro
CaT upstroke time (ms) 266 ± 5 (n = 3) 234 ± 2 (n = 3)
CaT 50% decay time (ms) 186 ± 23 (n = 3) 255 ± 4 (n = 3)
CaT 50 (ms) 452 ± 12 (n = 3) 490 ± 2 (n = 3)

The NKX2.5-GFP-labeled ESC line was employed for in vitro and ex vivo (i.e., kidney patch) analyses. All measurements made by optical mapping during 1-Hz pacing.