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. 2018 Jul 7;7(6):397–405. doi: 10.1302/2046-3758.76.BJR-2017-0140.R2

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© 2018 Author(s) et al.

This is an open-access article distributed under the terms of the Creative Commons Attributions licence (CC-BY-NC), which permits unrestricted use, distribution, and reproduction in any medium, but not for commercial gain, provided the original author and source are credited.

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graphic file with name bonejointres-07-397-g004.jpg — a) Histomorphometry on von Kossa and van Gieson-stained slides shows that there is a statistically significant increase in osteoid surface/bone surface (OS/BS) and osteoid volume/bone volume (OV/BV) in Phospho1^-/- fractured bone sections; b) analysis of toluidine blue-stained sections showed a significant increase in the osteoblast count and surface area in Phospho1^-/- fracture sites; c) analysis of tartrate-resistant acid phosphatase-stained sections showed that there was no statistically significant difference in the osteoclast count between the two groups (N.Ob, number of osteoblasts; T.Ar, tissue area; Ob.s, osteoblast surface; N.Oc, number of osteoclasts; Oc.s, osteoclast surface).