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. 2018 Jul 6;19:168. doi: 10.1186/s12882-018-0977-3

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Apoptotic cells in HK-2 after transfection observed by TUNEL. a HK-2 observed under light microscopy (200×). b HK-2 observed under a fluorescent microscope, apoptotic cells are fluorescent green (200×). c TUNEL-positive cells observed under light microscopy, and apoptotic cells are dark brown (200×). d TUNEL-positive cells counted and expressed as means ± SD. TUNEL: terminal-deoxynucleoitidyl transferase mediated nick end labeling. ***p < 0.001, relative to the control group. ^##p < 0.01, relative to the LPS group