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. 2018 Jul 6;11:92. doi: 10.1186/s13045-018-0635-z

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — CD25 high expression on GFP-NKG2D-z CAR T cells is essential for enrichment in the presence of IL-2. a One representative donor NKG2D CAR T cells expressed high level of CD25 and low level of CD25 by depleting human CD25 with magnetic separation on day 5 (4 days post transduction). b Percentages of CD25^high and CD25^low expressing GFP+ (NKG2D CAR) cells, presented as the mean ± SD, were derived from three independent donors during a 3-week culture period in the presence of IL-2 (50 IU/ml). c The kinetic monitoring of CD25^high and CD25^low expressing GFP (NKG2D CAR) expression on one representative donor of three in the presence of IL-2