Table 5.
Strains used in this study
| Strains | Relevant characteristic(s) | Reference |
|---|---|---|
| E. coli JM109 | recA1 endA1 gyrA96 thi-1 hsdR17 e14-(mcrA-) supE44 relA1 ∆(lac-proAB)/F’ [traD36 proB + laclq lacZ∆M15] | Stratagene |
| C. glutamicum strains | ||
| ATCC13032 | Wild-type strain, biotin auxotrophic | ATCC |
| Lys5 | l-lysine producer with the mutation of pyc gene | [5] |
| JL-6 (or CICIM B1031) | C. glutamicum AECr SDr FPs Metl, derived from strain ATCC13032, deposited in China Information Center of Industrial Microbial (CICIM) | CICIM |
| JL-61 | Deletion of pck gene in strain JL-6 chromosome | This work |
| JL-62 | Deletion of odx gene in strain JL-6 chromosome | This work |
| JL-63 | Deletion of malE gene in strain JL-6 chromosome | This work |
| JL-64 | Replacement of the natural promoter of malE gene with the tuf promoter in strain JL-6 chromosome | This work |
| JL-65 | Deletion of pck and odx genes in strain JL-6 chromosome | This work |
| JL-66 | Replacement of pck gene by ppc cassette in strain JL-6 chromosome | This work |
| JL-67 | Replacement of odx gene by pyc cassette in strain JL-6 chromosome | This work |
| JL-68 | Replacement of pck and odx gene by ppc and pyc cassette respectively in strain JL-6 chromosome | This work |
| JL-68∆gltA | Deletion of gltA gene in strain JL-68 chromosome | This work |
| JL-68∆ramA | Deletion of ramA gene in strain JL-68 chromosome | This work |
| JL-68∆P1gltA (or JL-69) | Deletion of 160 bp fragment contained P1 promoter of gltA gene in strain JL-68 chromosome | This work |
| JL-68∆P12gltA | Deletion of 540 bp fragment contained P1 and P2 promoter of gltA gene in strain JL-68 chromosome | This work |
| JL-68PdapA-L1 gltA | Replacement of the natural promoter of gltA gene with the dapA-L1 promoter in strain JL-68 chromosome | This work |
| JL-68∆prpC1 | Deletion of prpC1 gene in strain JL-68 chromosome | This work |
| JL-68 ∆prpC2 | Deletion of prpC2 gene in strain JL-68 chromosome | This work |
| JL-68∆prpC1∆prpC2 | Deletion of prpC1 and prpC2 genes in strain JL-68 chromosome | This work |
| JL-68∆gltA∆prpC1 | Deletion of prpC1 gene in strain JL-68∆gltA chromosome | This work |
| JL-68∆gltA∆prpC2 | Deletion of prpC2 gene in strain JL-68∆gltA chromosome | This work |
| JL-68∆gltA∆prpC1∆prpC2 | Deletion of prpC1 and prpC2 genes in strain JL-68∆gltA chromosome | This work |
| JL-68∆gltA∆prpC1prpRG977A | G977A mutation in prpR of JL-67∆gltA∆prpC1 chromosome | This work |
| JL-69PdapA-L1 gdh | Replacement of the natural promoter of gdh gene with the dapA-L1 promoter in strain JL-69 chromosome | This work |
| JL-69Ptac gdh | Replacement of the natural promoter of gdh gene with the tac promoter in strain JL-69 chromosome | This work |
| JL-69Ptac-M gdh | Replacement of the natural promoter of gdh gene with the tac-M promoter in strain JL-69 chromosome | This work |
| JL-69Ptuf gdh | Replacement of the natural promoter of gdh gene with the tuf promoter in strain JL-69 chromosome | This work |
| JL-69Psod gdh | Replacement of the natural promoter of gdh gene with the sod promoter in strain JL-69 chromosome | This work |