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. 2018 Jul 6;50:36. doi: 10.1186/s12711-018-0407-9

Table 2.

qPCR conditions

prpn lpo1 belt1 belt2 belt3 belt_b1 belt_d2
Annealing temperature (°C) 61 63 63 61 63 63 65
SYBR green 6.25 6.25 6.25 6.25 6.25 6.25 6.25
UNG 0.075 0.075 0.075 0.075 0.075 0.075 0.075
Forward (5 µM) 0.5 0.3 0.5 0.3 0.3 0.2 0.2
Reverse (5 µM) 0.5 0.5 0.5 0.5 0.5 0.3 0.2
Template 2.5 2.5 2.5 2.5 2.5 2.5 2.5
H2O 2.675 2.875 2.675 2.875 2.875 3.175 3.275