Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jul 6;13:111. doi: 10.1186/s13023-018-0855-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 6 — Immunoreactivity of IgG anti-laminin 332 autoantibodies with the GnT-III and GnT-V forms of laminin 332. a Schematic representation of GnT-III and GnT-V laminin 332. b Sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of the GnT-III (lane 2) and GnT-V (lane 3) proteins shows migration of α3 and γ2 chains at around 160 kDa and β3 chain at around 135 kDa. Weight markers are shown in lane 1. c Immunoreactivity of mucous membrane pemphigoid autoantibodies with the GnT-III and GnT-V isoforms of laminin 332. The two isoforms of laminin 332 were electrophoretically separated by 8% SDS-PAGE, transferred to nitrocellulose and immunoblotted with rabbit anti-human laminin 332 polyclonal antibody (lane 1), anti-laminin 332 mucous membrane pemphigoid patients‘sera (lanes 2–4), normal human sera (lane 5) and normal rabbit sera (lane 6). Anti-laminin 332 autoantibodies reacted in a similar manner with the two isoforms of laminin 332 (GnT-III and GnT-V)