Table 1.

The experimental design of the hemagglutination assay

Days	Groups	Treatment/activities
Day 0	Groups I–V	Sensitization of animals intraperitoneally with 0.2 mL of 5% SRBCs
Day 1–Day 5	Drug administration:
	Group I	Tween-80 (control)
	Group II	Nanoparticulate curcumin at 5 mg/kg
	Group III	Nanoparticulate curcumin at 10 mg/kg
	Group IV	Free curcumin at 5 mg/kg
	Group V	Free curcumin at 10 mg/kg
Day 6	Groups I–V	Collection of blood via ocular puncture (primary antibody titre).
Day 7	Groups I–V	Challenge of animals with 0.1 mL of 5% SRBC intraperitoneally.
Day 8– Day 12	Groups I–V	Treatment (same as that on days 1–5 with each group and its corresponding treatment).
Day 13	Groups I–V	Collection of blood via ocular puncture (secondary antibody titre)