Table 2.

The experimental design of the delayed-type hypersensitivity assay

Days	Groups	Treatment
Day 0	Groups I, II, III, IV and V	Sensitization of each animal with 0.2 mL of 5% SRBC intraperitoneally.
Day 1–Day 5	Group I	Tween 80 (control)
	Group II	Nanoparticulate curcumin at 5 mg/kg
	Group III	Nanoparticulate curcumin at 10 mg/kg
	Group IV	Free curcumin at 5 mg/kg
	Group V	Free curcumin at 10 mg/kg
Day 6	Groups I–V	Measurement of paw thickness
	Groups I–V	Challenge with 0.04 mL of 5% SRBC on left footpad and 0.04 mL of normal saline on right footpad (control)
	Groups I–V	Measurement of paw thickness after 4 h
Day 7–Day 8	Groups I–V	Measurement of paw thickness at 24 and 48 h.
Day 8–Day 12	Groups I–V	Treatment (same as that of days 1–5)
Day 13	Groups I–V	Collection of whole blood via ocular puncture into EDTA bottles for extraction of plasma sample for analyses of haematological parameters. Sacrificing of animals by cervical dislocation and extraction of liver and spleen.