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. 2018 Jul 6;11:397. doi: 10.1186/s13071-018-2984-4

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — MCP-1, IFN-γ and IL-10 were significantly elevated in the serum of ITV-immunized PD-1^-/- mice. Serum samples were collected from immunized WT (n = 5) and PD-1^-/-mice (n = 5) at the indicated times after the final immunization and the concentrations of cytokines were measured serially by a CBA. Three individual experiments were performed. The data are presented as the mean ± SD. Data were compared with the two-way ANOVA. Abbreviation: n, number of samples