Figure 2.

Ultrasound Treatment Setup

(A) Injection of a DNA and microbubble mixture into the bone tunnel sites. The ultrasound probe is placed adjacent to the needle for optimal visualization of the injected mixture. (B) Fluoroscopic imaging of needle placement within the bone tunnel sites is shown. Arrows denote the inserted needles. (C) Ultrasound contrast agent imaging during injection of the plasmid and microbubbles mixture is shown. Arrow denotes the inserted needle in the tunnel. (D) Ultrasonographic visualization of the injected mixture (marked with dashed line) within the bone tunnel during sonoporation is shown.