Figure 4.

Reporter Gene Expression in Mini-pig Reconstruction Sites following Ultrasound-Mediated Gene Delivery

(A) Flow cytometry analysis of the percentage of GFP-positive cells isolated from bone tunnel sites, with or without ultrasound treatment, 5 days after treatment. (B) Mean fluorescence intensity per cell in GFP-positive cells isolated from bone tunnels is shown. (C–E) Percentage of GFP-positive cells positive for CD29 (C), CD44 (D), and CD90 (E). n = 3 per experimental group; *p < 0.05; **p < 0.01; no US, group in which no ultrasound was used; US, group in which ultrasound was used. Data are represented as mean ± SEM.