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. Author manuscript; available in PMC: 2018 Oct 30.
Published in final edited form as: Nat Nanotechnol. 2018 Apr 30;13(7):610–617. doi: 10.1038/s41565-018-0113-3

Figure 6. Axial positioning of ligand controls CD45 exclusion.

Figure 6

CD45 exclusion and pY colocalization on NP clusters (inter-NP spacing 40 nm). Large clusters c127s40d50 (cluster size 127, density 50 NP/µm2): Fluorescence images with line profiles on (a) 2D and (b) 3D surfaces, (c) pY and CD45 intensity ratio of the cluster over surrounding area. Small clusters c4s40d4 (cluster size 4, density 4 NP/µm2): Fluorescence images with line profiles on (d) 2D and (e) 3D surfaces, (f) pY and CD45 intensity ratio. The experiment was repeated 3 times independently with similar results. The insets of a, d, are zoom-in AFM images of NP clusters. Size bar in fluorescence images: 5 µm. Data in c, f, are means ± s.d., each point representing a cluster in cell, n =8 cells from 2 independent experiments for each data set (Mann–Whitney test, two-sided).The difference is not significant if p-value > 0.05 (italic).