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Materials for transfection of rice protoplasts with Raichu-OsRac1. a Healthy Oc cells. b Materials required for transfection on a clean bench. c Preparation of a funnel with a nylon mesh filter. d Cellulase treatment on an orbital shaker. Incubate the cells with shaking at 50 rpm. e Filtration of cellulase-treated Oc cells. f, Precipitated cells after centrifugation with a swinging bucket rotor
