Fig. 8.

Quantification of IBA1+ and ED1+ cells in the optic tract of the brain. Mean ± SEM numbers of a IBA1+ and c ED1+ cells across the lower, middle, and upper regions of the optic tract in control uninjured optic nerve, compared to 3 and 7 days following partial optic nerve transection. n = 6 for control, n = 5 for 3 and for 7 days. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001. Representative images of b IBA1+ and d ED1+ cells at high magnification, indicated by “>,” in the lower region of the optic tract in control uninjured optic nerve, and 3 and 7 days following partial optic nerve transection. Scale bar for b–d: 5 μm. e Representative images of IBA1+ and ED1+ cells at low magnification, in the lower middle and upper regions of the optic tract in control uninjured optic nerve, and 7 days following partial optic nerve transection. Scale bar: 100 μm