Figure 4.

Confocal Imaging of HEK293 cells treated with amidated rhodamine pro-fluorophore, 1 c. [a–c] Images of a set of PLE-expressing HEK293 cells treated with 1 c and the DNA-specific fluorescent dye, Hoechst. [d–f] Images of a set of wild-type HEK293 cells treated under the same conditions as panels a–c. The scale bar shown in the lower right of each image is 20 μm. Images are representative of 4 independent replicates.