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. Author manuscript; available in PMC: 2018 Sep 28.
Published in final edited form as: Nature. 2018 Mar 28;556(7699):118–121. doi: 10.1038/nature25985

Figure 4. Interaction between RodA and its class B Penicillin Binding Protein (PBP2).

Figure 4

a, Evolutionary covariation map showing 19 evolutionary couplings between RodA and PBP2. b, Graphical representation of evolutionary covariation. c, Mutations in RodA/bPBP interface result in morphological abnormalities. Mutations were made in E. coli RodA, with T. thermophilus numbering in parentheses. Data representative of 2 independent experiments. Scale bar 5 μm. d, Model for peptidoglycan biogenesis. Lipid II is flipped across the membrane by MurJ, and polymerized and crosslinked by a complex of a SEDS protein (RodA) and bPBP, or by a bifunctional aPBP.