Table 2. Sequence specific primers of rhizobacterias, Azospirillum brasilense (AB) and Azotobacter chroococcum (AC) for qRT-PCR.
Primer | Sequence (5′-3′) | GenBank accession | Description |
---|---|---|---|
AB-4 | Forward: CAAGGGCACCATCCCGAC | X51500.1 | A. brasilense NifH gene |
Reverse: CTGCTGCTCCTCCGACT | |||
AC-2 | Forward: GTGACCCGAAAGCTGACTCC | EU693338.1 | A. chroococcum nifH gene |
Reverse: CCACCTTCAGCACGTCTTCC |