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. Author manuscript; available in PMC: 2018 Jul 8.
Published in final edited form as: Nature. 2018 Jun 6;558(7709):313–317. doi: 10.1038/s41586-018-0199-7

Extended Data Figure 2. siRNA validation experiments.

Extended Data Figure 2

HeLa (a,b,e) or U2OS (c,d) cells were treated with the indicated siRNA for 24 hrs prior to fixation and immunostaining. Scale bar is 20 μm. Single-cell quantification of immunofluorescence data was used to generate the histograms. a, n=7,152 cells for siControl and n=4,051 cells for siCyclin A2. b, n=7,772 cells for siControl and n=3,916 cells for siSkp2. c, n=7,003 cells for siControl and n=8,589 cells for siCyclin F. d, n=9,393 cells for siControl and n=7,871 cells for siEmi1. e, n=64,574 cells for siControl and n=53,338 cells for siCyclin E1,2.