Table 2.
Modified PCR cycling for HPV detection analysis from FFPE tissues.
| Process | Temperature (°C) | Duration | Number of cycles |
|---|---|---|---|
| SB01/SB02 primers | |||
| Pre-denaturation | 95 | 5min | 1 |
| Denaturation | 95 | 1min | 10 |
| Annealing | 42 | 1min | |
| Extension | 72 | 1min | |
| Denaturation | 95 | 20s | 30 |
| Annealing | 42 | 40s | |
| Extension | 72 | 1min | |
| Final extension | 72 | 10min | 1 |
| MY09/11 primers | |||
| Pre-denaturation | 95 | 5min | 1 |
| Denaturation | 95 | 15s | 40 |
| Annealing | 50 | 1min | |
| Extension | 72 | 1min | |
| Final extension | 72 | 10min | 1 |
| GP5/6 primers | |||
| Pre-denaturation | 95 | 10min | 1 |
| Denaturation | 95 | 30s | 40 |
| Annealing | 50 | 40s | |
| Extension | 72 | 30s | |
| Final extension | 72 | 10min | 1 |