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Comparison among several primer combination PCRs and Abbott m2000sp system method. In brackets are the primer pairs and their combination. nPCR refers to nested PCR.
| One step PCR (MY09/11) | nPCR (SB01/02 + MY09/11) |
nPCR (MY09/11 + GP5/6) | Double-nPCR (SB01/02 + MY09/11+ GP5/6) |
Abbott m2000rt system | |
|---|---|---|---|---|---|
| Positive | 4 (2.99%) | 58 (43.3%) | 74 (55.2%) | 129 (96.3%) | 134 (100%) |
| Negative | 130 (97.01%) | 76 (56.7%) | 60 (44.8%) | 5 (3.7%) | 0 (0%) |
| Total | 134 | 134 | 134 | 134 | 134 |
