Figure 4.

Minocycline alone reduces GNTI-induced scratching behavior, with no further reductions after the addition of MA. Mice were pretreated with minocycline (20 mg/kg) with or without MA at LI4 and LI11 20 min before GNTI administration. Mice administered GNTI alone served as controls. The number of scratching bouts was counted for 40 min in each group. Scratching behavior was reduced by similar amounts in both minocycline-treated groups; MA did not further improve the reduction in GNTI-induced scratching behavior. Between-group comparisons for each group were performed by ANOVA, followed by the Dunnett's test (**P < 0.01; ***P < 0.001; the GNTI-only group served as the reference group).