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. 2018 Jul 2;9:1518. doi: 10.3389/fimmu.2018.01518

Figure 3.

Figure 3

Activated astrocytes, microglia, and damaged neurons in the spinal cord of experimental autoimmune encephalomyelitis (EAE) mice treated with glycyrrhizin (GL). Immunohistochemical staining for glial fibrillary acidic protein (GFAP), Iba1, and NeuN in thoracic spinal cord sections of naive mice and EAE mice treated with either NaCl or GL obtained at day 25 post-immunization. 1: dorsal column; 2: central canal. (A) GFAP-positive cells. Scale bars were 10 µm for low magnification and 50 µm for high magnification. (B) Iba1-positive cells. Scale bars were 10 µm for low magnification and 20 µm for high magnification. (C) NeuN-positive cells. Scale bars were 10 µm for low magnification and 20 µm for high magnification. (D–F) The number of GFAP-positive cells, Iba1-positive cells, and NeuN-positive cells in spinal cords. At least six serial thoracic spinal cord sections were analyzed from each mouse, and six mice were included in each group. Data are shown as the mean ± SEM. ###P < 0.001 vs naive group; *P < 0.05, ***P < 0.001 vs EAE + NaCl group, statistical significance (P) was determined by one-way ANOVA with Bonferroni’s test.