Table 1.

Clinical features of experimental autoimmune encephalomyelitis (EAE) mice with glycyrrhizin (GL) treatment.

	Strategy I: pre-onset stage				Strategy II: onset and peak stages				Strategy III: peak stage
Group	No. with EAE/Total	Incidence	Mean maximal score (mean ± SEM)	Disease onset (day, mean ± SEM)	No. with EAE/Total	Incidence	Mean maximal score (mean ± SEM)	Disease onset (day, mean ± SEM)	No. with EAE/Total	Incidence	Mean maximal score (mean ± SEM)	Disease onset (day, mean ± SEM)
EAE	10/10	100	3.15 ± 0.15	12.30 ± 0.37	12/12	100	2.96 ± 0.20	13.17 ± 0.36
EAE + NaCI	10/10	100	3.05 ± 0.13	12.10 ± 0.31	12/12	100	3.04 ± 0.16	12.92 ± 0.42	9/9	100	3.17 ± 0.15	11.56 ± 0.18
EAE + 10 mg/kg GL	5/5	100	2.70 ± 0.20	12.60 ± 0.24	8/8	100	2.38 ± 0.32	12.38 ± 0.32	9/9	100	2.78 ± 0.19	11.78 ± 0.15
EAE + 25 mg/kg GL	10/10	100	2.90 ± 0.15	12.50 ± 0.40	7/14	50	0.86 ± 0.27***, ###, $$	15.71 ± 1.23*, #, $	9/9	100	2.61 ± 0.18#	11.56 ± 0.18
EAE + 50 mg/kg GL	5/5	100	2.30 ± 0.20*, #	12.20 ± 0.37	6/8	75	1.19 ± 0.33**, ###, $	14.50 ± 0.67#, $	9/9	100	2.44 ± 0.26#	12.00 ± 0.24

“EAE”: EAE induction without treatment. “EAE + NaCl”: EAE induction with 0.9% NaCl treatment. “EAE + GL”: EAE induction with 10, 25, or 50 mg/kg GL treatment. *P < 0.05, ***P < 0.001 vs EAE group; ^#P < 0.05, ^###P < 0.001 vs EAE + NaCl group, ^$P < 0.05, ^$$P < 0.01 vs EAE + 10 mg/kg GL group, using Mann–Whitney U test. The results shown are one experiment or representative of two independent experiments.