Table 2.
Excised copy numbers and excision rates of MSSA476 prophages at selected optical densities.
| Prophage | OD600 | Excised prophage copy number (copies attP/copy gyrA) |
Excision rate (copies attB/copy gyrA) | |
|---|---|---|---|---|
| exDNA | gDNA | |||
| ΦSa3ms | 0.35 | 0.3801 ± 0.050 | 0.109 ± 0.009 | 4.64 × 10-4 ± 4.52 × 10-5 |
| 0.7∗ | 0.3377 ± 0.064 | 0.095 ± 0.005 | 4.80 × 10-4 ± 1.10 × 10-5 | |
| 1.0 | 0.2653 ± 0.021 | 0.1370 ± 0.013 | 5.05 × 10-4 ± 2.73 × 10-5 | |
| O/N | 0.2563 ± 0.014 | 0.1230 ± 0.001 | 9.58 × 10-4 ± 2.14 × 10-5 | |
| ΦSa4ms | 0.35 | 2.220 ± 0.110 | 0.0840 ± 0.006 | 1.51 × 10-4 ± 2.47 × 10-5 |
| 0.7∗ | 1.257 ± 0.229 | 0.0647 ± 0.001 | 9.91 × 10-5 ± 3.13 × 10-6 | |
| 1.0 | 0.4270 ± 0.030 | 0.0767 ± 0.008 | 8.44 × 10-5 ± 1.14 × 10-5 | |
| O/N | 0.2073 ± 0.019 | 0.0700 ± 0.008 | 8.75 × 10-5 ± 8.15 × 10-6 | |
Asterisk “∗” indicates culture density representative of samples from extra-chromosomal DNA sequencing. O/N, overnight culture. Values are displayed as mean ± SEM.