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. 2018 Jul 2;9:1515. doi: 10.3389/fimmu.2018.01515

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Copyright © 2018 Zhao, Jia, Wang, Chen, Huang, Xu, Zhao, Xiang, Yang and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cobalt protoporphyrin (CoPP) treatment induces HO-1 high expression in BM-derived immature dendritic cells (imDCs). Untreated, CoPP-pretreated, or SnPP-pretreated imDCs and untreated or CoPP-pretreated mDCs were used in the experiments. (A) HO-1 expression was measured by Western blotting. Anti-β-actin was used as a loading control. The corresponding densitometric analyses are shown as bar graphs. The mean ± SD is reported (*P < 0.05, n = 3 per group). (B) Immunofluorescent staining of HO-1 was performed on each group of dendritic cells (DCs). Confocal images shown are representative of three experiments with similar results (original magnification, 600×; red, CD11c; green, HO-1; blue, nuclei). (C) Flow cytometry analysis of HO-1 was performed on each group of DCs. Gmean values are shown. Similar results were obtained in three independent experiments. (D) The phenotype (MHC-II, CD80, and CD86) of each group of DCs was analyzed by flow cytometry. The results shown here are representative of three independent experiments.