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. 2018 Jul 2;9:1515. doi: 10.3389/fimmu.2018.01515

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Copyright © 2018 Zhao, Jia, Wang, Chen, Huang, Xu, Zhao, Xiang, Yang and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Upregulation of heme oxygenase-1 renders immature dendritic cells (imDCs) refractory to lipopolysaccharide (LPS)-induced maturation. Untreated, cobalt protoporphyrin-pretreated, or SnPP-pretreated imDCs were incubated with LPS to induce maturation for 24 h. Untreated imDCs without LPS stimulation were used as controls. The expression of MHC-II, CD40, CD80, and CD86 was assessed by FACS analysis. (A) Representative FACS results of three independent experiments. Numbers in quadrants indicate the percentage of positive cells. (B) Quantitative assessment of the percentages of MHC-II/CD40, MHC-II/CD80, and MHC-II/CD86 double-positive cells. The data shown are means ± SD (n = 3 per group; **P < 0.01).