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. 2018 Jul 2;9:1515. doi: 10.3389/fimmu.2018.01515

Figure 3.

Figure 3

Upregulation of heme oxygenase-1 enhances the ability of immature dendritic cells (imDCs) to inhibit allogeneic T-cell proliferation stimulated by anti-CD3/CD28 Abs. CFSE-labeled lymphocytes from C57BL/6 mice were stimulated with anti-CD3ε and anti-CD28 Abs (2 µg/ml) to achieve profound cell proliferation. Graded doses of untreated imDCs, cobalt protoporphyrin-pretreated imDCs, SnPP-pretreated imDCs, or mDCs from BALB/c mice were added to the lymphocyte stimulation system at different ratios (1:4, 1:8, 1:16, and 1:32). Three days later, CD4+ and CD8+ T-cell proliferation was separately evaluated by FACS analysis. (A,B) Representative FACS results of three independent experiments for CD4+ (A) and CD8+ (B) T-cell proliferation. Numbers within the graph denote the percentage of proliferated cells. (C,D) The average percentages of proliferated CD4+ (C) and CD8+ (D) T cells. Data are shown as mean ± SD (n = 3 per group). (E,F) Supernatants were collected after 48 h from the lymphocyte stimulation system at a DC/T cell ratio of 1:16 and analyzed by ELISA to determine the levels of IFN-γ (E) and IL-10 (F). Data are shown as means ± SD (n = 3 per group, *P < 0.05, **P < 0.01).