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. 2018 Jul 2;9:1515. doi: 10.3389/fimmu.2018.01515

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Copyright © 2018 Zhao, Jia, Wang, Chen, Huang, Xu, Zhao, Xiang, Yang and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Donor-derived immature dendritic cells (imDCs) are mainly located in recipient spleens after adoptive transfer. Untreated BALB/c mouse-derived imDCs (5 × 10⁶) labeled with CellVue Claret (red) were intravenously injected into C57BL/6 recipients. At 1, 7, and 14 days after the injection, mesenteric lymph nodes (A–C) and spleens (D–F) were harvested to detect the localization of allogeneic imDCs. After staining with DAPI (blue), tissue sections were observed under a confocal imaging system. The photographs shown are representative of three independent experiments.