Figure 7.

Adoptive transfer of donor-derived HO-1^hi immature dendritic cells (imDCs) is more efficient in suppressing the activation and proliferation of recipient lymphocytes. Untreated, cobalt protoporphyrin-pretreated, or SnPP-pretreated donor-derived imDCs (5 × 10⁶) were injected intravenously into recipient mice, and heart transplantation was performed 7 days later. As a control, recipient mice received no cell transfusion, and only an injection of the same volume of PBS. The splenocytes were isolated from normal C57BL/6 mouse spleens or recipient spleens collected 7 days after heart transplantation (n = 3 per group). (A) The expression of IFN-γ in CD4⁺ or CD8⁺ T cells was detected by flow cytometry. Representative FACS results of three independent experiments are shown. (B) mixed leukocyte reaction (MLR) was performed using irradiated donor splenocytes as stimulator cells and CFSE-labeled recipient splenocytes as responder cells. The proliferation of recipient CD4⁺ and CD8⁺ T cells was measured by FACS after a 7-day MLR. Representative FACS results of three independent experiments are shown.