Figure 8.
Donor-derived HO-1hi immature dendritic cells (imDCs) are more potent in attenuating anti-donor humoral responses in cardiac allograft recipients. Untreated, cobalt protoporphyrin-pretreated, or SnPP-pretreated BALB/c mouse-derived imDCs were adoptively transferred to C57BL/6 recipients 7 days before cardiac transplantation. As a control, recipient mice received no cell transfusion, and only injection with the same volume of PBS. The sera were collected from normal C57BL/6 mice or recipient mice on day 7 after heart transplantation (n = 3 per group). The circulating anti-donor IgG and IgM levels were detected by flow cytometry using BALB/c splenocytes as target cells. Data expressed are the Gmean values. Representative FACS results of three independent experiments are shown.