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Methods that measure co-translational mRNA decay. (A) Splinted PCR utilizes an oligonucleotide specific to both the mRNA and the adapter to ensure gene-specific amplification of decapped transcripts. (B) Direct ligation methods take advantage of the free 5′-monophosphate to directly capture decapped and cleaved transcripts for sequencing. (C) 5PSeq captures both capped and decapped transcripts for sequencing and comparison.
