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Melatonin significantly enhances AMPK activation during osteoblast differentiation of human MSCs. (A) Human MSCs were treated with the indicated concentrations of melatonin for 24 h under ODM. (B) Cell were treated with the indicated concentrations of melatonin (100 μM) and compound C for 24 h under ODM. Western blot analysis was performed with the specified antibodies as described in Materials and Methods. Representative data from multiple experiments are shown.
