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Relative mRNA expression level of TLR4 was detected by qPCR. Fold of change of TLR4 mRNA expression level was measured at different time intervals after silica exposed to U937-differentiated macrophages when compared to time 0 (which is set as 1). Total RNA amount of each sample was normalized by expression level of β-actin. Data was expressed as mean ± standard deviation (S.D.) of 3 replicates.
