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. 2018 Jun 14;15(10):986–991. doi: 10.7150/ijms.24715

Figure 2.

Figure 2

Detection of expression level of TLR4, MyD88 and TIRAP by Western blot analysis. U937-differentiated macrophages were exposed to silica and incubated for 24 h in the presence or absence of pre-incubation of TAK-242 before total protein was harvested. Then, the protein expression level of TLR4, MyD88 and TIRAP was detected by primary antibodies and respective secondary antibodies followed by enhanced chemiluminescence detection and image capture by X-ray film. Total protein amount of each sample was normalized by expression of β-actin. The image was a representative of three independent trials.