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Release of IL-1β, IL-6, IL-10 and TNFα was measured by ELISA. After the U937-differentiated macrophages were exposed to silica for 24 h in the presence or absence of TAK-242 pre-treatment, the release of IL-1β (A), IL-6 (B), IL-10 (C) and TNFα (D) was measured by ELISA. Data was expressed as mean ± standard deviation (S.D.) of 3 replicates. Significant difference was indicated by * p < 0.05 and ** p < 0.01.
