Effect of EETs on [Ca2+]i in HEK293 cells and HEK293 cells stably expressing human GPR40 (HEK293 + GPR40).
A and B, effect of various concentrations of the EETs on [Ca2+]i in HEK293 cells (A) and HEK293 + GPR40 cells (B) (data expressed in Δ relative fluorescence units (ΔRFU) per μg of protein). C and D, effect of 11,12-EET on [Ca2+]i fluorescence over time in HEK293 cells (C) and HEK293 + GPR40 cells (D) (data shown in RFU × 10−3). E and F, effect of 14,15-EET on [Ca2+]i fluorescence over time in HEK293 cells (E) and HEK293 + GPR40 cells (F) (data shown in RFU × 10−3). Each value represents the mean ± S.E. for n = 4. veh, vehicle.