Figure 3.
Effect of extracellular [Ca2+]i on EET activity in HEK293 cells expressing human GPR40. A–C, comparison of the activity of various concentrations of EET regioisomers and GPR40 agonist GW9508 in the presence of 1.26 mm extracellular calcium (A), in the absence of extracellular calcium (B), and in the absence of extracellular calcium plus 50 μm EGTA (C). D, effect of 1 μm 11,12-EET on [Ca2+]i with time in the presence of 1.26 mm extracellular Ca, in the absence of extracellular Ca, and in the absence of extracellular calcium plus 50 μm EGTA. Each value represents the mean ± S.E. for n = 4. veh, vehicle.