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. 2018 May 18;293(27):10675–10691. doi: 10.1074/jbc.RA117.001297

Figure 5.

Figure 5.

Effect of the GPR40 antagonist GW1100 (10 μm) and GPR40 siRNA on the increase in [Ca2+]i by 11,12-EET, 14,15-EET, and the GPR40 agonist GW9508 in human GPR40-expressing HEK293 cells. A, effect of GW1100 on the activity of GW9508. B, effect of GPR40 siRNA on the activity of GW9508. C, effect of GW1100 on the activity of EETs. D, effect of GPR40 siRNA on the activity of EETs. E and F, Western blotting (E) and densitometry (F) of GPR40 protein levels and knockdown in human GPR40-expressing HEK293 cells treated with control and GPR40 siRNA for 48 h. A and C, each value represents the mean ± S.E. for n = 4. B and D, each value represents the mean ± S.E. for n = 8. F, each value represents the mean ± S.E. for n = 4. * indicates p < 0.05, compared with control siRNA. veh, vehicle.