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m6A-Seq validation. A and B, meRIP-qPCR analysis of input (pre-meRIP) and m6A levels of Esrrb (A) and c-myc (B) mRNAs from WT and Gsk-3 DKO ESCs. Bars labeled WT or DKO, mRNA levels before meRIP. Bars labeled WT-m6A and DKO-m6A, mRNA levels after Me-RIP. All meRIP-qPCR analyses are relative to Gapdh mRNA levels. Each experiment shown was performed in triplicate. Error bars, S.D. *, statistical significance between the indicated groups (p < 0.05, unpaired t test, two-tailed).
